Ischemic Stroke Model in Cynomolgus Monkeys

Ischemic Stroke Model in Cynomolgus Monkeys | MCAO Validation

Huateng Bio's cynomolgus monkey MCAO stroke model replicates human thromboembolic events via endovascular embolization or open craniotomy. Features DSA-guided autologous thrombus delivery, penumbra quantification, and neurobehavioral deficit assessment for neuroprotection drug development. AAALAC-certified protocols.

Model Name

Middle Cerebral Artery Occlusion (MCAO) Stroke Model

Animal Strains

Cynomolgus Monkeys (Macaca fascicularis)

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Model Description
Ischemic stroke, accounting for 80% of clinical stroke cases, is caused by cerebral artery occlusion leading to irreversible neuronal damage through ischemic cascade reactions. Our MCAO primate models replicate human cerebrovascular pathology with:
• High clinical relevance: MCA is the most frequent occlusion site in human stroke
• Controlled ischemia: Permanent/transient occlusion mimicking thromboembolic events
• Translational endpoints: Penumbra formation & neurobehavioral deficits

Applications
• Neuroprotection drug development
• Reperfusion injury mechanism studies
• Therapeutic time window evaluation
• Advanced imaging technology validation (e.g. DSA/MRI)

Modeling Methods

1. DSA-Guided Endovascular Embolization
• Procedure: Femoral artery cannulation → autologous venous thrombus delivery to MCA under digital subtraction angiography (DSA) guidance
• Key advantages:

Non-cranial approach (prevents intracranial infection)

Precise ischemia-reperfusion control (tPA compatibility)

Low postoperative mortality

2. Open Craniotomy with Electrocoagulation
• Procedure: Permanent MCA occlusion via direct electrocoagulation under surgical visualization
• Key advantages:

High reproducibility of infarct volume

Penumbra formation confirmed by TTC staining

Compatible with multimodal physiological monitoring

Validation & Testing

Category	Protocols
Imaging Analysis	DSA angiography ∙ MRI/CT cerebral blood flow mapping
Behavioral Assays	Modified Neurological Severity Score (mNSS) ∙ Limb placement test
Histopathology	TTC staining (infarct volume) ∙ H&E staining ∙ GFAP/Iba1 immunohistochemistry

Technical Comparison

Method	Advantages	Limitations
DSA Embolization	∙ Minimally invasive ∙ Reperfusion-capable ∙ Clinically translational	∙ Inter-animal variability in occlusion sites
Craniotomy Electrocoagulation	∙ High infarct consistency ∙ Definitive penumbra generation	∙ Craniectomy-related complications ∙ No reperfusion option

Ischemic Stroke Model in Cynomolgus Monkeys | MCAO Validation